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FLOWJO COMPENSATION MATRIX IMPORT SOFTWARE
FLOWJO COMPENSATION MATRIX IMPORT SERIES

In essence, AutoSpill uses iterations of linear regressions to get the best compensation or unmixing matrices, and removes the need for gating the positive and negative fractions of your controls. introduced AutoSpill, which resolved the gating issue raised in this blogpost. Add additional tubes to Tube list if required, load tubes and record data for each tube until all data required has been collected.Edit January 19, 2023: The 2021 Nature paper by Carlos P. The sample tube holder returns to the loading position.

Select Record to save the data and wait for the saving process to finish.

Alternatively, select the Threshold button from the tool bar and move the mouse to desired threshold position in the desired plot. Choose the channel for threshold setting and manually enter a value in Threshold tab.

Adjust the threshold settings if required to remove unnecessary signal noise by selecting the Threshold tab in the Acq.

The recommended settings are only for reference. Note: Optimize the gain settings according to your own experimental goals. Setting window or use the Gain Control button on the toolbar directly on the plot where the data appears during data collection.

Adjust gain settings for the different channels in Gain tab in the Acq.

In Acquisition panel, select sample flow rate and type in desired acquisition settings for Events to Display, Events to Record, and Time to Record.

Place the sample tube in sample tube holder and select Run to load the sample.

Refer to Importing and Exporting Compensation in Compensation module of the Cytoflex IFU for more information. Select desired option for importing compensation matrix and gain settings. Select Import from Library or Import and locate the compensation matrix file (.comp) to import.

Import compensation matrix to apply compensation values to experiment if required by selecting Compensation Matrix from the Settings menu to bring up the compensation matrix table.

In the Gain tab, select Recommended in cases where you do not specify your own default parameter gain settings. Setting window and display cytometer gains and threshold settings. Setting button on the left side panel to open the Acq.

Right click the Statistics table and select Statistics Setting and check desired header, statistics and populations of interest. Select the Statistics button for the toolbar to display statistics window.You can change color and names of gates by double clicking the color or name in population hierarchy window. Select the Population Hierarchy button from the toolbar to display the population hierarchy.Select the heading area of the plot to assign the gates to the required plots.To create gates, use the gating control buttons on the toolbar or right click the plot and select the gate type required.Select the axis name on the plot to change which channel is displayed. Explore the tool bar buttons to create plots in the experiment workspace.Type in reagent name in the label column if desired. Select the desired channel signals in the checkbox. Select Set Channel from the Settings menu.Select New Experiment in the File menu, specify the file path and name the experiment or accept the default naming Exp_YYYMMDD-1 and select Save.The experimenter can choose which one to execute first.

FLOWJO COMPENSATION MATRIX IMPORT SOFTWARE

Liquid Handling and Scheduling SoftwareĬytExpert software separates color compensation and sample acquisition experiments into separate files designated.

CytExpert Software for the CytoFLEX Platform.

Plate Loader Options for the CytoFLEX Platform.

FLOWJO COMPENSATION MATRIX IMPORT SERIES

CytoFLEX Violet-Blue-Red Series Upgrades.Labware for Liquid Handling Instruments.Biomek NGeniuS Reaction Vessel, 24 Well, 64/Box.Lysing, Fixative, and Permeabilizating Reagents.RESOURCE Contract Manufacturing Services.HIAC 9703+ Pharmaceutical Particle Counter.Biomek NGeniuS Next Generation Library Prep System.Cell Counters, Sizers and Media Analyzers.

Air Particle Counters for Cleanroom and Environmental Monitoring.